Evaluation of the glutathione concentration in whole blood of dairy Holstein cows

The objective of this study was to evaluate the concentrations total (T-GSH), oxidised (GSSG) and reduced (GSH) glutathione in blood dairy cows, assess relationships phase number lactation, milk production, body condition score (BCS) selected biochemical parameters on its concentrations. We analysed 79 samples whole from Holstein cows. concentration assessed by spectrophotometric enzymatic method. dataset shows mean T-GSH, GSSG GSH 803 ± 22 µmol/l, 23 2.5 µmol/l 757 27 respectively. GSH/GSSG ratio 95 17. lactation had a significant impact levels T-GSH GSH, but BCS no effect. Dry cows higher (938 44 µmol/l) than fresh (713 46 peak (785 45 significantly lower (618 (719 dry (827 43 µmol/l). On basis regression analysis, affected mainly liver function energy metabolism. Glutathione as marker oxidation stress seems be promising tool monitoring health welfare herd, yet intensive research field remains necessary.